リボヌクレアーゼE

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リボヌクレアーゼE
識別子
EC番号	3.1.26.12
CAS登録番号	76106-82-6
データベース
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB構造	RCSB PDB PDBj PDBe PDBsum
検索
PMC	articles
PubMed	articles
NCBI	proteins
	テンプレートを表示

リボヌクレアーゼE(Ribonuclease E、EC 3.1.26.12)は、以下の化学反応を触媒する酵素である^[1]^[2]^[3]^[4]^[5]^[6]。

一本鎖RNAのアラニン及びウラシルの豊富な領域をエンドヌクレアーゼ的に切断する。

この酵素は細菌の持つリボヌクレアーゼで、リボソームRNA（9Sから5SrRNA）の処理に関与する。

出典

^ Feng, Y., Vickers, T.A. and Cohen, S.N. (2002). “The catalytic domain of RNase E shows inherent 3′ to 5′ directionality in cleavage site selection”. Proc. Natl. Acad. Sci. USA 99 (23): 14746-14751. doi:10.1073/pnas.202590899. PMC 137490. PMID 12417756.
^ Ehretsmann, C.P., Carpousis, A.J. and Krisch, H.M. (1992). “Specificity of Escherichia coli endoribonuclease RNase E: in vivo and in vitro analysis of mutants in a bacteriophage T₄mRNA processing site”. Genes Dev. 6 (1): 149-159. doi:10.1101/gad.6.1.149. PMID 1730408.
^ Cormack, R.S., Genereaux, J.L. and Mackie, G.A. (1993). “RNase E activity is conferred by a single polypeptide: overexpression, purification, and properties of the ams/rne/hmp1 gene product”. Proc. Natl. Acad. Sci. USA 90 (19): 9006-9010. doi:10.1073/pnas.90.19.9006. PMC 47490. PMID 8415644.
^ Vanzo, N.F., Li, Y.S., Py, B., Blum, E., Higgins, C.F., Raynal, L.C., Krisch, H.M. and Carpousis, A.J. (1998). “Ribonuclease E organizes the protein interactions in the Escherichia coli RNA degradosome”. Genes Dev. 12 (17): 2770-2781. doi:10.1101/gad.12.17.2770. PMC 317140. PMID 9732274.
^ Steege, D.A. (2000). “Emerging features of mRNA decay in bacteria”. RNA 6 (8): 1079-1090. doi:10.1017/S1355838200001023. PMC 1369983. PMID 10943888.
^ Callaghan, A.J., Grossmann, J.G., Redko, Y.U., Ilag, L.L., Moncrieffe, M.C., Symmons, M.F., Robinson, C.V., McDowall, K.J. and Luisi, B.F. (2003). “Quaternary structure and catalytic activity of the Escherichia coli ribonuclease E amino-terminal catalytic domain”. Biochemistry 42 (47): 13848-13855. doi:10.1021/bi0351099. PMID 14636052.

外部リンク

Ribonuclease E - MeSH・アメリカ国立医学図書館・生命科学用語シソーラス（英語）

[1] Feng, Y., Vickers, T.A. and Cohen, S.N. (2002). “The catalytic domain of RNase E shows inherent 3′ to 5′ directionality in cleavage site selection”. Proc. Natl. Acad. Sci. USA 99 (23): 14746-14751. doi:10.1073/pnas.202590899. PMC 137490. PMID 12417756.

[2] Ehretsmann, C.P., Carpousis, A.J. and Krisch, H.M. (1992). “Specificity of Escherichia coli endoribonuclease RNase E: in vivo and in vitro analysis of mutants in a bacteriophage T₄mRNA processing site”. Genes Dev. 6 (1): 149-159. doi:10.1101/gad.6.1.149. PMID 1730408.

[3] Cormack, R.S., Genereaux, J.L. and Mackie, G.A. (1993). “RNase E activity is conferred by a single polypeptide: overexpression, purification, and properties of the ams/rne/hmp1 gene product”. Proc. Natl. Acad. Sci. USA 90 (19): 9006-9010. doi:10.1073/pnas.90.19.9006. PMC 47490. PMID 8415644.

[4] Vanzo, N.F., Li, Y.S., Py, B., Blum, E., Higgins, C.F., Raynal, L.C., Krisch, H.M. and Carpousis, A.J. (1998). “Ribonuclease E organizes the protein interactions in the Escherichia coli RNA degradosome”. Genes Dev. 12 (17): 2770-2781. doi:10.1101/gad.12.17.2770. PMC 317140. PMID 9732274.

[5] Steege, D.A. (2000). “Emerging features of mRNA decay in bacteria”. RNA 6 (8): 1079-1090. doi:10.1017/S1355838200001023. PMC 1369983. PMID 10943888.

[6] Callaghan, A.J., Grossmann, J.G., Redko, Y.U., Ilag, L.L., Moncrieffe, M.C., Symmons, M.F., Robinson, C.V., McDowall, K.J. and Luisi, B.F. (2003). “Quaternary structure and catalytic activity of the Escherichia coli ribonuclease E amino-terminal catalytic domain”. Biochemistry 42 (47): 13848-13855. doi:10.1021/bi0351099. PMID 14636052.

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